Analysis of clinical characteristics of lung adenocarcinoma patients with exon 21 L858R deletion mutation / 肿瘤研究与临床

Objective:To explore the clinical characteristics of lung adenocarcinoma patients with exon 21 L858R deletion mutation.Methods:The data of 112 patients who were diagnosed with lung adenocarcinoma and positive genetic mutations in the People's Hospital of Xinjiang Uygur Autonomous Region from January 2015 to December 2018 was retrospectively analyzed, and the patients were divided into the exon 21 L858R deletion mutation group (52 cases) and the non-exon 21 L858R deletion mutation group (60 cases). The clinical characteristics, imaging characteristics, expressions of tumor markers and smoking history of patients were compared between the two groups.Results:There was no statistical difference in the gender, age and ethnicity between the exon 21 L858R deletion mutation group and the non-exon 21 L858R deletion mutation group (P values were 0.488, 0.238 and 0.191). There was no statistical difference in the imaging features (including primary tumor site, lobulation, burr, pleural depression and small vacuoles) between the two groups (all P > 0.05). There was no statistical difference in the expressions of tumor markers (including carcinoembryonic antigen, squamous cell carcinoma antigen, neuron-specific enolase, cytokeratin 19 fragment, and gastrin-releasing peptide precursor) between the two groups (all P > 0.05). There were 20 patients (38.5%, 20/52) with smoking history in the exon 21 L858R deletion mutation group, and 4 patients (6.7%, 4/60) with smoking history in the non-exon 21 L858R deletion mutation group, the difference between the two groups was statistically significant (χ 2 = 4.182, P = 0.041). Conclusions:There is no significant difference in clinical characteristics, imaging features and expressions of tumor markers between the patients with exon 21 L858R deletion mutation and the patients without exon 21 L858R deletion mutation. Smoking may be the influencing factor of exon 21 L858R deletion mutation.

Analysis the methylation of caveolin-2 gene in peripheral blood of uygur pigeon feeders / 中国医师进修杂志

study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2. Methods Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2. Results CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (P>0.05). The methylation rates of each site between the three groups were compared in pairs, and showed no statistical differences (P > 0.05). Conclusions Whether CAV2 gene methylation has any effect on the pulmonary pathogenesis and pulmonary fibrosis process of uygur pigeon feeders remains to be further studied.

Analysis the methylation of caveolin-2 gene in peripheral blood of uygur pigeon feeders / 中国医师进修杂志

Objective@#To study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2.@*Methods@#Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2.@*Results@#CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (P > 0.05). The methylation rates of each site between the three groups were compared in pairs, and showed no statistical differences (P > 0.05).@*Conclusions@#Whether CAV2 gene methylation has any effect on the pulmonary pathogenesis and pulmonary fibrosis process of uygur pigeon feeders remains to be further studied.